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丹那唑抑制大鼠线粒体呼吸链复合酶I 导致原代肝细胞坏死(PDF)

《现代预防医学》[ISSN:1003-8507/CN:51-1365/R]

期数:
2015年20期
页码:
3752-3755
栏目:
实验技术及其应
出版日期:
2015-10-20

文章信息/Info

Title:
Danazol induces necrosis of primary cultured rat hepatocytes by inhibition of mitochondrial respiratory chain complex I
作者:
孟华1王军2
1.河南南阳医学高等专科学校第一附属医院,河南 南阳 473058;
2.西安交通大学第一附属医院急诊科,陕西 西安 710061
Author(s):
MENG Hua* WANG Jun
Nanyang Medical College, First Affiliated Hospital, Nanyang, Henan 473058, China
关键词:
关键词:丹那唑肝细胞线粒体复合酶I细胞毒性
分类号:
R319
DOI:
-
文献标识码:
A
摘要:
摘要:目的 研究丹那唑对大鼠原代肝细胞的损伤作用与机制。方法 原代大鼠肝细胞贴壁培养4 h,加入丹那唑0,50,100,200 μmol/L,4 h和24 h后采用乳酸脱氢酶法测定细胞活力并检测三磷酸腺苷(ATP)水平,同时提取线粒体,分别测5个呼吸链复合酶(complex I~V)活性。培养液中加入20 mmol/L果糖促进糖酵解,观察果糖对丹那唑细胞毒性的影响。结果 丹那唑50,100 μmol/L处理4 h和24 h对细胞几乎没有杀伤作用,但可致ATP显著降低;200 μmol/L处理4 h和24 h均可导致细胞坏死。果糖可对抗丹那唑4 h具杀伤作用,但对24 h杀伤作用无效。丹那唑(50~200 μmol/L)显著抑制线粒体呼吸链复合酶I活性,但不影响complex Ⅱ~V活性。结论 丹那唑选择性抑制线粒体呼吸链复合酶I活性而导致ATP生成减少并诱发细胞坏死。

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备注/Memo

备注/Memo:
基金项目: 陕西省自然科学基金(2013JM4049)肝性脑病的中枢5-HT机制
作者简介: 孟华(1976-),女,硕士,副教授,研究方向:脑血管药理学
通讯作者: 王军,E-mail:wangj0205@126.com
更新日期/Last Update: 2015-10-28